As an owner of the business, doing your research is a must. You have to be aware of suspicious activities that could initiate fraud.
According to the research, there is always a pattern when it comes to online fraud. It can be multiple purchases from the same IP but from different cards or uncommon large purchases. Whether your payment processor has his security checks in place, sometimes a fraudulent order can slip through.
So, once you start to recognize the pattern have detailed research and training on it, it becomes easier to recognize those. Though the single point of activity may not make it suspicious enough to identify as a fraud, multiple data points with a trained model can certainly help.